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. 2001 Apr 11;86(2):275–285. doi: 10.1016/S0092-8674(00)80099-9

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© 1996 Cell Press

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Quantitation of Cell and Nuclear Morphology

SKP1/SKP1 wild-type, skp1-3/skp1-3 and skp1-4/skp1-4 homozygous mutants, and skp1-3/skp1-4 heteroallelic diploids were grown to logarithmic phase at 25°C and shifted to 37°C for 3 and 6 hr, and nuclear and bud morphology were scored. The criteria used for each morphologic class scored are shown schematically above the columns. The numbers shown represent percentages of the total cells scored (far right column). Asterisk, in this population of cells, 67% had the nucleus at the neck and 33% had the nucleus spanning the neck (n = 203).