Table 1.

Detailed information about the experimental protocol on the magnetic bead-based microfluidic platform.

Step	Procedure	Sample volume	On-chip operation condition
1	Load virus-bound magnetic beads in the sample loading/mixing chamber.	5 μl
	Load serum sample in the sample loading/mixing chamber.	100 μl
	Deflect the membranes of the micropump to block the sample inside the sample purification chamber.		20 psi for the micropump
2	Mix the bio-sample with the magnetic beads for 5 min in the incubation process by using the membrane-type micromixer.		20 psi and 1.37 Hz for the micromixer
3	Pump the well-mixed sample into the sample purification chamber at a flow rate of 620 μl/min.		20 psi and 35 Hz for the micropump
4	Turn on the magnetic field to collect the IgM/IgG-bound magnetic beads onto the surface of the sample purification chamber for 1 min by using the microcoils array.		150 mA for microcoils
5	Load the washing buffer into the sample loading chamber.	200 μl
	Pump the washing buffer through the purification chamber at a flow rate of 620 μl/min for washing.		20 psi and 35 Hz for the micropump
	Re-suspend the purified samples into a volume of 100 μl and transport the sample into the sample loading/mixing chamber.	100 μl	20 psi and 35 Hz for the micropump
6	Pump half of the purified sample to the sample chamber at a flow rate of 620 μl/min.	50 μl	20 psi and 35 Hz for the micropump
7	Load developing antibody (anti-human IgG-FITC) in the sample loading/mixing chamber.	100 μl
	Mix the developing antibody with the purified samples for 2 min in the incubation process by using the membrane-type micromixer.		20 psi and 1.37 Hz for the micromixer
8	Pump the well-mixed sample into the sample purification chamber at a flow rate of 620 μl/min.		20 psi and 35 Hz for the micropump
9	Apply the DC current into the microcoils array to trap the magnetic complexes within the sample purification chamber for 1 min.		150 mA for microcoils
	Load the washing buffer in the sample loading chamber and then flow the buffer through the purification chamber at a flow rate of 620 μl/min.	300 μl	20 psi and 35 Hz for the micropump
	Re-suspend the purified magnetic complexes into a volume of 5 μl and transport the complexes to the sample detection chamber at a flow rate of 182.5 μl/min.	5 μl	20 psi and 35 Hz for the micropump
10	Pump the purified sample in the sample chamber to sample loading/mixing chamber at a flow rate of 620 μl/min.	50 μl	20 psi and 35 Hz for the micropump
	Load developing antibody (anti-human IgM-R-PE) in the sample loading/mixing chamber.	100 μl
	Mix the developing antibody with the purified samples for 2 min in the incubation process by using the membrane-type micromixer.		20 psi and 1.37 Hz for the micromixer
11	Pump the well-mixed sample into the sample purification chamber at a flow rate of 620 μl/min		20 psi and 35 Hz for the micropump
12	Apply the DC current into the microcoils array to trap the sandwich-like magnetic bead complexes within the sample purification chamber for 1 min.		150 mA for microcoils
	Load the washing buffer in the sample loading chamber and then flow the buffer through the purification chamber at a flow rate of 620 μl/min.	300 μl	20 psi and 35 Hz for the micropump
	Re-suspend the purified sandwich-like magnetic bead complexes into a volume of 5 μl and transport the complexes to the sample detection chamber at a flow rate of 182.5 μl/min.	5 μl	20 psi and 35 Hz for the micropump
13	The fluorescent signal is excited and is analyzed by an optical detection module.