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Western blot (a) 1 μg, 10 μg of PpSP15 and BSA were probed with mouse anti-PpSP15 antisera (1:2000 dilution) and (b) 1 μg, 10 μg of PpSP15, 7.2 μg of in-house Pichia pastoris X33 HCPs, BSA, and Cygnus HCP were probed with goat anti-P. pastoris HCP antibody (1:1000 dilution). M: SeeBlue Plus2 protein.
