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HPLC chromatogram of the crude sample extracted from the root of liquorice: 1, glycyrrhizin. HPLC conditions: reversed-phase Symmetry C18 column (150 mm×3.9 mm i.d., 5 μm); column temperature, 30 °C; mobile phase, methanol–water–acetic acid (65:34:1 (v/v)); flow rate, 1.0 ml min−1; diode array detection at 251 nm; injection volume, 20 μl.
