Fig. 2.

Illustrations and pictures of the integrated isothermal device. (A) The structure of the disposable micro-reactor. The disposable micro-reactor has a reaction chamber and 5-mm optical pathway. The reaction chamber is 5 mm-long, 2 mm-wide and 5 mm in height. (B) The structure of the base apparatus. The base apparatus is made with PMMA to create a detection pathway and sliding track. It has two slices of flexible heaters on the top and on the bottom sides. The light source fibers and the optical sensor are lined on the detection pathway and put close (about 10 mm) to each other. The reaction box is 60 mm-long, 50 mm-wide and 14 mm high. The detection pathway is 60 mm-long, 10 mm-wide, 5 mm-height and the sliding track is 35 mm-long, 6 mm-wide, 5 mm-height. (C) Picture of the disposable micro-reactor. This micro-reactor is just a home-made product. The disposable micro-reactor is constructed from two components of PMMA and glass slide cover. It has a reaction chamber and a 5-mm optical pathway without heaters or sensors. (D) Picture of the base apparatus. For the LAMP reaction, the reaction chamber is filled with 25-μl reagent and then sealed by gluey aluminum foil. The disposable micro-reactor component can be inserted into the base apparatus and the HBV LAMP reaction can be started under isothermal condition. This system can provide appropriate reaction conditions for HBV LAMP DNA amplification.