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. 2013 Apr 27;23(3):357–364. doi: 10.1016/j.sbi.2013.03.009

Figure 1.

Figure 1

S. cerevisae Mediator Complex. (a) Subunit organization of Mediator. Head module subunits are colored in blue; those of the Middle module are in green and those of the Tail module are in ochre. (b) Genes encoding the Mediator Head module were assembled from multiple DNA progenitors (left) into a single multigene expression construct (right, top) for insertion by Tn7 transposition (Tn7L, Tn7R) into the MultiBac baculovirus to produce the Head module in infected insect cells (right, bottom). (c) X-ray crystal structure of the recombinant Mediator Head module showing Med17 (blue), Med11 (purple), Med22 (green), Med6 (yellow), Med8 (red), Med18 (cyan), and Med 20 (orange). The Head consists of three distinct domains, fixed jaw, movable jaw and neck (left). The neck domain is arranged in a novel multihelical bundle (right) [30••].