Table 4.
Examples of commercially available affinity tags and respective purification supports based on biological ligands.
| Biological ligands | Affinity solid support and supplier | Affinity tag | Capacity (mg/ml) |
|---|---|---|---|
| Peptides and proteins B | Glutathione sepharose (GE Healthcare) Glutathione superflow (Clontech) Glutathione HiCap matrix (Qiagen) Pierce glutathione agarose/magnetic beads (Thermo Scientific) Glutathione agarose and magnetic agarose beads(Sigma) |
GST tag | 10 10 20 40/5–10 5–10/15 |
| Calmodulin resin (VWR) MagneZoom™—CAM (bioWorld) Calmodulin resin (G Biosciences) |
CBP | 3 na 3 |
|
| S protein Agarose (Novagen-EMD Millipore) | S-tag | na | |
|
StrepTactin™ (GE Healthcare) Strep-Tactin superflow/magnetic beads (Qiagen) Strep-Tactin superflow (Novagen-EMD Millipore) |
Strep-tag II | 6 9/0.3 3 |
|
| IgG agarose (GE Healthcare) IgG agarose (Sigma) |
Protein A/G tag |
2 1 |
|
| Anti-FLAG M1, M2 resin (Sigma-Aldrich) Anti-FLAG antibody agarose/magnetic beads (Clontech) |
Flag-tag | 0.6 na/1.3 |
|
| EZview™ red Anti-myc resin (Sigma) Anti-c-myc agarose (Sigma-Aldrich) Anti c-myc antibody agarose (Clontech) Anti-Myc-tag mAb-magnetic beads (MBL) Pierce c-myc-Tag IP (Thermo Scientific) |
c-myc tag | 0.05 0.2 0.5 na na |
|
| T7 Tag® antibody agarose (EMD Millipore) T7 tag antibody agarose (Genetex) Anti-T7 tag® antibody agarose (Abcam) |
T7-tag | 0.3 0.5 na |
|
| Pierce Anti-HA agarose (Thermo Scientific) Pierce Anti-HA magnetic beads (Thermo Scientific) Ezview™ anti-HÁ affinity gel (Sigma) Anti-HÁ affinity matrix (3F10) (Roche) Anti-HA-tag mAb-magnetic beads (MBL) |
HA tag | 150 nmol/ml 10 μg/mg 0.4 9 nmol/ml na |
|
| Softag immune affinity resin (Neoclone) | Softag 1/2/3 |
0.3 | |
| Carbohydrates | Amylose resin/magnetic beads (New England Biolabs) | MBP | 7–10/0.01 |
| Cellulose (Sigma) | Cellulose binding domain | 15 | |
| Chitin agarose (New England Biolabs) | Chitin binding domain | 3 |
na—data not available.