Table 1.

The pilot experiment of lung slices model using ribavirin. 250 μm thick lung slices were prepared as described in Section 2.3. The lung slices were infected with 200 μl of 10⁵ PFU/ml PR8 virus. After 2 h incubation on 37 °C, the viruses were discarded and the lung slices were washed twice with PBS. Then six concentrations of ribavirin starting from 200 μM and with 3-fold serial dilution were added. NA activities and IP-10 levels in culture supernatants were determined 48 h post infection as described in Sections 2.7, 2.8.

Plates	S/B-NAa	S/B-IP-10b	Z′-NAc	Z′-IP-10d	NA-EC50 (μM)e	IP-10-EC50 (μM)f
1	8.3	10.2	0.67	0.73	32.5	21.8
2	6.2	6.7	0.53	0.68	25.4	17.8
3	9.3	8.6	0.63	0.58	28.3	20.6
4	7.8	7.3	0.71	0.64	17.7	14.2

^aS/B-NA: Signal to background for NA activity. Refer to ratio of NA signal in infected control wells to negative control wells.

^bS/B-IP-10: Signal to background for IP-10 release. Refer to ratio of IP-10 in infected control wells to negative control wells.

^cZ′-NA: Z′ factor for NA inhibition.

^dZ′-IP-10: Z′ factor for IP-10 inhibition.

^eNA-EC₅₀: 50% effective concentration determined by NA inhibition.

^fIP-10-EC₅₀: 50% effective concentration determined by IP-10 inhibition.