Figure 2.

PCK1 regulates glycolysis in cultured ccRCC cells.

Notes: (A) 769-P and Caki-1 cells with stably knocked down PCK1 or the negative control were or were not treated with 2-DG (2.5 mM). The numbers of cells were counted every 24 h. The bars represent the means±SEM (n=3). (B–G) PCK1 regulates glucose uptake (B), lactate production (C), extracellular acidification rate (ECAR) (D), oxygen consumption rate (OCR) (E, F), and 6-¹⁴CO₂ production from 6-¹⁴C- glucose (G) in 769-P and Caki-1 cells. The bars represent the means±SEM (n=3). (*P<0.05, **P<0.01, Student’s t-test).