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. 2009 Aug 12;145(2):312–320. doi: 10.1016/j.virusres.2009.07.024

Fig. 1.

Fig. 1

Subcellular localization of BICP27 in BHV-1 infected MDBK cells and transiently transfected cells. (A) MDBK cells were infected with BHV-1 at MOI of 0.1 immediately after transfection with pECFP-L23. 16 h after infection immunofluorescence staining of BICP27 was performed using antipeptide serum 50. Immunofluorescence photomicrograph of BICP27, the corresponding phase-contrast image and ECFP-L23 location photomicrographs are shown. Arrows indicated the nucleoli. (B) Subcellular localization of BICP27 and ECFP-L23. Twelve micrograms of protein was applied to lanes 1–5. The antibodies for BICP27 and ECFP were indicated on the right margin. Lane C, cytoplasmic fraction; lane NP, nucleoplasmic fraction; lane No, nucleolar fraction; lane N, nuclear fraction; lane W, whole cell extract. (C) Immunofluorescence analysis of COS-7 cells expressing BICP27. Cells transfected with pcDNA3.1-BICP27 were fixed 24 h post-transfection and immunofluorescent assay was carried out to detect the expression and subcellular localization of BICP27. Immunofluorescence photomicrograph (left) and the corresponding phase-contrast photomicrograph (right) are shown. Arrows indicated the nucleoli. Each image is representative of the vast majority of the cells observed.