Table 1.

Primers for constructing recombinant plasmids and deletion mutants.

Plasmids’ name	Forward primer	Reverse primer
pcDNA3.1–BICP27	CGGAATTCATGGCGGACCCCGAGATCGC	GCTCTAGATTACGGAAGATCTCGTTGCAGTAAA
pBICP27–EYFP	CGGAATTCATGGCGGACCCCGAGATCGC	CGGGATCCCGGAAGATCTCGTTGCAGTAAA
pEYFP–BICP27	CGGAATTCATGGCGGACCCCGAGATCGC	CGGGATCCTTACGGAAGATCTCGTTGCAGTAAA
pBICP27–dEYFP	CGGGATTCATGGTGAGCAAGGGCGAGGA	CGGGATCCCTTGTACAGCTCGTCCATGC
pBICP27–tEYFP	CGGGATTCATGGTGAGCAAGGGCGAGGA	CGGGATCCCTTGTACAGCTCGTCCATGC
paa1–50–EYFP	CGGAATTCATGGCGGACCCCGAGATCGC	CGGGATCCCAGCTTGCGCTTGCGAACCT
paa1–85–EYFP	CGGAATTCATGGCGGACCCCGAGATCGC	CGCGGATCCACGCGAGCCCGCCGCGCCG
paa1–91–EYFP	CGGAATTCATGGCGGACCCCGAGATCGC	CGCGGATCCCGCGGCCGCCGCGGCCGCA
paa1–100–EYFP	CGGAATTCATGGCGGACCCCGAGATCGC	CGCGGATCCGCGGGCTGTCTTCGGCGCG
paa1–214–EYFP	CGGAATTCATGGCGGACCCCGAGATCGC	CGGGATCCATGAGCGTCTCCCAGGTGAC
paa101–400–EYFP	CGGAATTCATGGGAGAGCAGCGGTCCCGCGG	CGACCGGTGGATCCCGGAAG
paa92–400–EYFP	CGGAATTCATGAGGCGCCCGCGCCGAAGACA	CGACCGGTGGATCCCGGAAG
paa86–400–EYFP	CGGAATTCATGCGGCCGCGGCGGCCGCGCAG	CGACCGGTGGATCCCGGAAG
paa81–400–EYFP	CGGAATTCATGCGGCGGGCTCGCGTGCGGCC	CGACCGGTGGATCCCGGAAG
NLS-del	CGCCGGAGGTGCTTCGGCGG	CGGCCGCGGCGGCCGCGCAG
NoLS-del	CACGCGAGCCCGCCGCGCCG	CAGCCCGCCGGAGAGCAGCG
NLS + NoLS-del	CGCCGGAGGTGCTTCGGCGG	CAGCCCGCCGGAGAGCAGCG
ptEYFP	CGGAATTCATGGTGAGCAAGGGCGAGGA	GAAGATCTTTGTACAGCTCGTCCATGC
paa81–97–tEYFP	AATTCATGCGGCGGGCTCGCGTGCGGCCGCGGCGGCCGCGCAGGCGCCCGCGCCGAAGG	GATCCCTTCGGCGCGGGCGCCTGCGCGGCCGCCGCGGCCGCACGCGAGCCCGCCGCATG
paa86–97–tEYFP	AATTCATGCGGCCGCGGCGGCCGCGCAGGCGCCCGCGCCGAAGACAG	GATCCTGTCTTCGGCGCGGGCGCCTGCGCGGCCGCCGCGGCCGCATG
paa81–84–tEYFP	AATTCATGCGGCGGGCTCGCG	GATCCGCGAGCCCGCCGCATG
pGL-gCp-Luc	TTAGATCTTGAACGGCTGCCGCCGAC	AAAAGCTTGGCGGACGCGCGGCGAGG