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. 2018 Jun 15;273:316–321. doi: 10.1016/j.snb.2018.06.069

Fig. 5.

Fig. 5

Clinical utility of CRISPR/dCas9-mediated biosensor. (A) Schematic of CRISPR/dCas9-mediated biosensor for ST diagnosis in ST and SFTS samples. (B) Highly sensitive and specific detection of ST-DNA with CRISPR/dCas9-mediated biosensor (blue circle) in six clinical samples compared to that of SMR biosensor alone (green circle). P1-3, human sera from ST patients as positives; N1-3, human sera from severe fever with thrombocytopenia syndrome (SFTS) patients as negatives. (C) Schematic of CRISPR/dCas9-mediated biosensor for SFTS diagnosis in ST and SFTS samples. (D) Highly sensitive and specific detection of SFTS-RNA with CRISPR/dCas9-mediated biosensor (blue triangle) in six clinical samples compared to that of biosensor alone (green triangle). P1-3, human sera from SFTS patients as positives; N1-3, human sera from ST patients as negatives. Error bars indicate standard deviation from the mean, based on at least three independent experiments. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).