Table 2.
Optical density measurements of ReproCHEK™ reagent (Synbiotics Corporation, San Diego, CA, USA) and internal (coyote) controls
| Positive relaxin control | Negative relaxin control | Female coyotes not-bred | Male coyotes | |
|---|---|---|---|---|
| Minimum | 0.051 | −0.001 | 0.004 | 0.001 |
| Maximum | 0.487 | 0.037 | 0.032 | 0.018 |
| Mean | 0.249 | 0.009 | 0.015 | 0.009 |
| Median | 0.252 | 0.006 | 0.014 | 0.009 |
| n | 63 | 63 | 13 | 8 |
Reagent positive and negative controls were included in each assay run; internal controls were randomly assigned. Values represent the parameters observed during 2000–2003 breeding seasons; NWRC facility, Millville, UT, USA. n: number of manufacturer's controls or non-pregnant coyote samples tested.