FIG. 4.

Partial sequence comparison of the C-terminal region from Human NEP (P08473), Bombyx mori NEP (BAB33300), endopeptidase sequence from Manduca sexta (AAO21504), Drosophila melanogaster NEP (NP_511056), Aplysia californica NEP (AAD51382), Human ECE-1 (NP_001388), Locusta migratoria ECE (AAN73018), Drosophila melanogaster ECE (NP_523417), Hydra vulgaris ECE (AAD46624). 1, ET-binding site; 2, active site HExxH; 3, zinc-binding consensus site ExxxD. The colors indicate the nature of the amino acids (red: acid; light-blue: base; dark-blue: hydrophilic; grey: hydrophobic.)