Fig. 1.

Phylogenetic analyses for N gene (A), and S gene (B) of FRCoVs. FRCoVs from America and Europe are indicated by red and blue boxes, respectively. Two genetic groups identified for S gene of American FRCoVs are represented by American-I and American-II. Numbers at the nodes indicate bootstrap support evaluated by 500 replicates. Recombination analyses of S gene of FRCoVs (C and D). The FECV1 strain from America was used as the query and compared with four strains: FRCoV_NL_2010 (NL), FSCV6(US), ferret063(Japan), and FRCoV4370(Japan). (C) SimPlot shows the genetic distance between query sequence and each reference sequence in different parts of the genome. (D) Bootscan plot shows phylogenetic relationship between query sequence and reference sequences in different parts of the S gene. Potential region for recombination event is highlighted by dash lines.