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. 2002 May 25;288(3):305–320. doi: 10.1006/jmbi.1999.2688

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Copyright © 1999 Academic Press. All rights reserved.

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The nucleotide composition at the bottom of stem 1 influences frameshifting. (a) Base-pairs at the bottom of stem 1 of pseudoknots of pKA11, 13 and 18 were changed using site-directed mutagenesis. The parental sequences present in stem 1 are boxed and the variant sequences are shaded. Also shown is pKA33, a variant of pKA18 with a destabilised stem 2. (b) RRL translation products synthesised in response to mRNAs derived from BamHI-digested pKA11, 13, 18 and mutant derivatives. Products were labelled with [³⁵S]methionine, separated on a SDS-15 % polyacrylamide gel and detected by autoradiography. The frameshifted (22, 28 or 85 kDa) and non-frameshifted (19 kDa) species are marked with arrows.

The nucleotide composition at the bottom of stem 1 influences frameshifting. (a) Base-pairs at the bottom of stem 1 of pseudoknots of pKA11, 13 and 18 were changed using site-directed mutagenesis. The parental sequences present in stem 1 are boxed and the variant sequences are shaded. Also shown is pKA33, a variant of pKA18 with a destabilised stem 2. (b) RRL translation products synthesised in response to mRNAs derived from BamHI-digested pKA11, 13, 18 and mutant derivatives. Products were labelled with [³⁵S]methionine, separated on a SDS-15 % polyacrylamide gel and detected by autoradiography. The frameshifted (22, 28 or 85 kDa) and non-frameshifted (19 kDa) species are marked with arrows.