Fig. 2.

Synergy between actinomycin D and nutlin-3a induces pro-caspase-1 (CASP1) expression. A. Levels of pro-caspase-1 (CASP1), p53, phospho-p53, p21, and loading control (HSC70) in A549 cells treated with A + N or CPT for indicated time. Con - mock-treated control. B. Changes in the levels of CASP1 mRNA, measured by semi-quantitative real-time PCR of RNA samples isolated from mock-treated A549 cells (Con) or from cells incubated for 30 h with actinomycin D (ActD), nutlin-3a (Nut), both substances A + N, or CPT. The mRNA level in the mock-treated population was defined as 1. Results represent mean and standard deviations from three biological replicates, *** p < .001 by two-tailed Student's t-test, treatment versus control. C. Levels in whole-cell lysates of pro-caspase-1 protein, p53 phosphorylated on Ser392 and loading control (HSC70). A549 cells were treated as in A for 48 h. The location of molecular weight markers is indicated.