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. 2003 Mar 15;308(1):13–22. doi: 10.1016/S0042-6822(02)00096-X

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Fig. 3 — Rescue and growth kinetics of rTGEV-Δ7 from cDNA in BHK-pAPN cells. (A) Scheme of pBAC-TGEV-RS-Δ7 coding for rTGEV-RS-Δ7 virus. The deletion Δ7 virus that includes 21 nt of TRS-7 and the first 17 nt of ORF-7 (outlined letters) is underlined. The core sequence is shown in bold. Acronyms as in Fig 1. (B) and (C) Virus titers of TGEV-Sptv-RS and TGEV-Sptv-RS-Δ7 (B) and TGEV-SC11-RS and TGEV-SC11-RS-Δ7 (C) recovered from BHK-pAPN cells transfected with plasmids pBAC-TGEV-Sptv-RS, pBAC-TGEV-Sptv-RS-Δ7, pBAC-TGEV-SC11-RS, and pBAC-TGEV-SC11-RS-Δ7, respectively. The recovered virus was amplified throughout the indicated passages, and the culture supernatants were titrated on ST cells. Mock, mock transfected cells. (D) and (E) Growth kinetics of cloned TGEV-Sptv-RS-Δ7 (D) and TGEV-SC11-RS-Δ7 (E) in cell culture, after infection at an m.o.i. of 5. Aliquots were collected at the indicated times and titrated on ST cells. Error bars represent standard deviations of the mean from four experiments.