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. 2003 Mar 15;308(1):13–22. doi: 10.1016/S0042-6822(02)00096-X

Fig. 4.

Fig. 4

Characterization of mRNAs and proteins of rTGEV-Δ7. (A) Northern blot analysis of viral mRNAs. Total RNA was isolated from BHK-pAPN cells infected with either rTGEVSptv-RS or rTGEV-Sptv-RS-Δ7. V−, noninfected BHK-pAPN cells. The position of viral mRNAs is indicated to the left. (B) Western blot analysis of lysates from BHK-pAPN cells infected with either rTGEV-Sptv-RS or rTGEV-Sptv-RS-Δ7 viruses. Cell extracts were obtained at 16 h p.i., resolved by 5 to 20% gradient SDS–PAGE, transferred to nitrocellulose membranes, and immunoblotted with monoclonal antibodies specific for S, N, M, and E, and with an antiserum specific for a protein 7 peptide (Garwes et al., 1989).