Fig. 2.

Western blot analysis of non-pathological (NP) and pathological (P) fAGP stained with the lectins Sambucus nigra agglutinin (SNAI) and Maackia amurensis agglutinin (MAA) that are specific for sialic acid α2-6)-linked and α2-3)-linked to galactose, respectively. In Western blots for both lectins (A), each lane was loaded with 2 μg purified protein. Non-pathological protein reacts strongly with both lectins when compared with the pathological protein. The signal recorded with the two lectins in the pathological pool is weak. For each lectin, the reactivity with SNAI and MAAI has been quantified by a densitometry and the quantification of the area under the peaks (B) confirms that for both lectins, the value corresponding to pathological fAGP is lower than that of non pathological fAGP.