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. 2020 Apr 3;20:108. doi: 10.1186/s12935-020-1157-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Downregulation of ZFPM2-AS1 suppressed malignant behaviors of SCLC cells. a qRT-PCR results of ZFPM2-AS1 level in SCLC cell lines and controls (16-HBE). b qRT-PCR results of ZFPM2-AS1 level following treating DMS-53 and SHP-77 cells with shRNAs targeting ZFPM2-AS1. c, d Colony formation and EdU analyses of DMS-53 and SHP-77 cell proliferation in response to ZFPM2-AS1 knockdown. In EdU assay, EdU-positive cells (stained in red) are cells that were proliferating, and DAPI stained cells (stained in blue) were total cells. e CCK-8 evaluated DMS-53 and SHP-77 cell viability when downregulating ZFPM2-AS1. f Transwell assay detected DMS-53 and SHP-77 cell invasion and migration after silencing ZFPM2-AS1. g Wound healing assay assessed DMS-53 and SHP-77 cell migration upon ZFPM2-AS1 silencing. *P < 0.05, **P < 0.01