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. 2020 Apr 3;20:108. doi: 10.1186/s12935-020-1157-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — ZFPM2-AS1 acted as sponge of miR-3612. a Both cytoplasmic and nuclear expression of ZFPM2-AS1 was measured via qRT-PCR after subcellular fractionation. b FISH probed the expression of ZFPM2-AS1 in cytoplasm and nucleus. c DIANA and starBase revealed the predicted miRNAs that might bind with ZFPM2-AS1. d RNA pull-down assay detected the enrichment of the indicated miRNAs in ZFPM2-AS1 probe group. e The binding site between miR-3612 and ZFPM2-AS1 was exhibited (upper). Luciferase activity of ZFPM2-AS1-WT or ZFPM2-AS1-MUT was monitored by luciferase reporter assay following NC mimics or miR-3612 mimics transfection (lower). f RIP assay examined the enrichment of ZFPM2-AS1 and miR-3612 in Anti-Ago2 group. g Overexpression efficiency of ZFPM2-AS1 in SHP-77 and DMS-53 cells was detected by qRT-PCR. h qRT-PCR evaluated the regulatory effects of ZFPM2-AS1 on miR-3612 expression in SHP-77 and DMS-53 cells. *P < 0.05, **P < 0.01, *** P < 0.001