Fig. 3.

EGR1 expression is dependent on ERK and PERK signaling. A-C) Primary astrocytes were mock-infected or infected with TC-83 (MOI 5) for 1 h and pre- and post-treated with either DMSO, ERKi (10 μM), p38MAPKi (10 μM), PI3Ki (500 nM), or PERKi (2.5  μM). At 18hpi total RNA was extracted and gene expression was determined using TaqMan assays for EGR1. Fold changes were calculated relative to 18S ribosomal RNA and normalized to mock samples using the ΔΔCt method. Data are expressed as the Mean ± SD (n = 3). *p-value ≤ 0.05, ***p-value ≤ 0.001, ****p-value≤ 0.0001. n.s. =not significant