Table 1.
Primers and probes for multiplex real-time detection of M. pneumoniae, C. pneumoniae, Legionella spp., and human DNA
| Primer/probe | Sequence (5′→3′) | Gene target | Product (bp) | Multiplex primer/probe final concentration |
|---|---|---|---|---|
| MP181-F | TTTGGTAGCTGGTTACGGGAAT | M. pneumoniae | 73 | 250 nmol/L |
| MP181-R | GGTCGGCACGAATTTCATATAAG | CARDS Tx (Kannan and Baseman, 2006) | 250 nmol/L | |
| MP181-P | HEX-TGTACCAGAGCACCCCAGAAGGGCT-BHQ1 | 100 nmol/L | ||
| CP-Arg-F | CGTGGTGCTCGTTATTCTTTACC | C. pneumoniae | 74 | 250 nmol/L |
| CP-Arg-R | TGGCGAATAGAGAGCACCAA | argR | 250 nmol/L | |
| CP-Arg-P | Quas670-CTTCAACAGAGAAGACCACGACCCGTCA-BHQ3 | 50 nmol/L | ||
| Pan-Leg-F | GGCGACCTGGCTTC | Legionella spp. | 230 | 125 nmol/L |
| Pan-Leg-R | TATGACCGTTGATTCGATACC | ssrA | 125 nmol/L | |
| Pan-Leg-P | FAM-ACGTGGGTTGC-BHQ1a | 25 nmol/L | ||
| RNase P-F | AGATTTGGACCTGCGAGCG | Human RNase P(Emery et al., 2004) | 62 | 250 nmol/L |
| RNase P-R | GAGCGGCTGTCTCCACAAGT | 250 nmol/L | ||
| RNase P-P | CalRd610-TTCTGACCTGAAGGCTCTGCGCG-BHQ2 | 50 nmol/L |
a
Locked nucleic acid probe.