Fig. 1.

The expression of surface molecules and binding of rgp120 to the human glioblastoma cell line D54 were determined by flow. (a) D54 cells did not express galactosyl ceramide (GalC) or the lipid-specific O11 antigen, but are positive for galactosyl sulfatide (Gal-S). As negative control an anti-coronavirus S mAb (Co) and as positive control the anti-MHC class I mAb W6/32 was used (MHC-I). (b) Detection of rgp120 bound to D54 cells using a polyclonal anti-gp120 antiserum (gp120). As control BSA instead of rgp120 was incubated with the cells under the same conditions (Co-BSA).