Fig. 1.

PF14 mediates efficient RNAi in reporter cell-lines. BHK-21 cells and HepG2 (5 × 10⁴) were seeded 24 h prior to experiments into 24-well plates. Cells were treated with PF14/siRNA nanocomplexes at three different concentrations at MRs 20:1, 25:1 and 30:1 for 4 h in serum-free medium followed by addition of serum to final concentration of 10% and incubated additionally for 20 h (A), and at MRs 30:1, 35:1 and 40:1 for 24 h in serum-containing medium (B). (C) For HepG2 cell-line, the cells were treated with PF14/siRNA nanocomplexes at different concentrations at MR30 in serum-containing medium. PF14 complexed with control (unrelated) siRNA was used at the highest siRNA dose and the highest MR in A, B and C. LF 2000® and LF RNAiMax® were used according to the manufacturer's protocol. Cells were lysed in 0.1% Triton X-100 and luciferase activity was measured. RNAi assay results are presented as percent of luciferase expression relative to untreated cells. The values represent the mean of at least three experiments performed in duplicate (mean ± SEM, n = 3).