Fig. 4.

PF14 forms definite nanocomplexes with siRNA that are stable upon drying and resuspension. Nanocomplexes, both freshly prepared and the in solid formulation, were studied using the Nanoparticle Tracking Analysis (NTA) system. A. The freshly prepared nanocomplexes measured in water. B. The freshly prepared nanocomplexes measured in OptiMEM® with 10% FBS, C. the solid formulation measured in water, D. the solid formulation measured in OptiMEM® with 10% FBS. The graphs on the middle panels show the size distribution of the particles with the particle size plotted on the x-axis and the particles concentration (E6/ml) plotted on the y-axis. The graphs on the rightmost panels are 3D plots (size vs. intensity vs. concentration), while the leftmost panels show the corresponding NTA video frames. In middle panels for samples B and D, the yellow curves represent the size distribution measured for the vehicle alone which is OptiMEM® with 10% FBS. D50: the diameter where half of the population lies below, D90: the diameter where 90% of the population lies below.