Figure 4.

Comparison of the NTDs Derived from the Closed Monomeric Conformation and from the Ring Structure Unveils Major Conformational Changes

(A) Superposition of Cα atoms 71 to 191 results in an rms deviation of 2.8 Å. Sites of major conformational movements are indicated with arrows. The NTD from the closed monomeric conformation is shown in red and the one from the octamer structure in yellow.

(B) Schematic overview of the two major conformational changes in VP40. An N-terminal loop (gray) and the C-terminal domain (gray) from the closed VP40 conformation must change their conformation to achieve octamerization. This is indicated by the ribbon drawing of the three regions involved; the potential movement of the two domains with respect to the N-terminal domain is highlighted by arrows.