Table 2.
LC–MS/MS analysis and identification of plasma proteins overexpressed in OVM-exposed mice
| Protein | Spot # | Accession # | Database MW/pI | Scaffold % probability protein identity | # Unique peptides | % Coverage | # Amino acids/total |
|---|---|---|---|---|---|---|---|
| Haptoglobin | 2100 | gi|8850219 | 38,752/5.9 | 100 | 28 | 41 | 143/347 |
| Haptoglobin | 1846 | gi|8850219 | 38,752/5.9 | 89 | 3 | 12 | 41/347 |
| Peroxiredoxin-2 | 1435 | gi|2499469 | 21,779/5.2 | 100 | 4 | 44 | 87/198 |
| Serum amyloid A2 | 1928 | gi|6755394 | 13,623 | 100 | 9 | 48 | 58/122 |
| Hemoglobin-alpha | 1928 | gi|6680175 | 15,085 | 100 | 4 | 31 | 44/142 |
Proteins of interest were excised from 2D-DIGE gels, digested with trypsin and analyzed by LC–MS/MS. Proteins were identified by matching unique spectra in the NCBI database for mus musculus using Scaffold software with dual search engines Mascot and X! Tandem.