Fig. 8.

ARG could not induce ROS generation but inhibited SVCV-induced ROS generation in EPC cells. (A) Workflow representing the experimental design followed in (B) and (C). Briefly, EPC cells were infected with SVCV for 2 h, then the medium was replaced with new medium containing 1.6 mg/L ARG. After incubation for 48 h, the cells were stained with 2′,7′–dichlorodihydrofluorescein diacetate (DCFH-DA) oxidant-sensitive probe for ROS detection. (B) Fluorescence observation of ROS in EPC cells treated with or without ARG. (C) The value of ROS fluorescence in EPC cells treated with or without ARG. Value represents the mean ± SD of three replicate samples. Significance between control and exposure groups are indicated by **P < 0.01, *P < 0.05.