Fig. 2.

APS inhibits IBV production. A. CEK cells were infected with IBV before treatment with different concentrations of APS(0,1, 5, 10, 20, or 30 μg/mL) for 24 h. Viral titers in supernatants were determined by the plaque formation assay and normalized to the value of the 0 group (set at 100%). B,C. Real-time qRT-PCR analysis of the levels of IBV genomic RNA as well as genomic and subgenomic mRNAs, asdetermined by analysis of the IBV 5′ UTR (B) and 3′UTR (C) in infected CEK cells following APS treatment. The differences between means were considered significant at *P < 0.05 and highly significant at **P < 0.01 when compared with the control groups (i.e. APS concentration, 0).