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. 2015 Dec 16;69(7):1166–1174. doi: 10.1038/sj.ki.5000246

Figure 3.

Figure 3

HGF activates c-Met and triggers multiple signaling pathways in endothelial cells, including PI3K–Akt, which is required for suppression of E-selectin. (a) HUVEC cells were pretreated with HGF (100 ng/ml) for 30 min before TNF-α (0.1 ng/ml) stimulation. At different time points after TNF-α stimulation, cell lysates were analyzed by immunoblotting for different molecules. (b) Pretreatment for 30 min with wortmannin (50 nM) and LY294002 (20 μM), specific chemical inhibitors for PI3K, diminished HGF's suppressive effect on E-selectin expression in HUVEC cells. (c) Pretreatment with a Stat3 inhibitor (160 μM PpYLKTK-mts, Calbiochem, San Diego, CA, USA), or with U0126 (10 μM) to block the Mek–Erk pathway, failed to override HGF suppression of E-selectin.