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. 2017 Dec 26;38:31–37. doi: 10.1016/j.mcp.2017.12.003

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Fig. 1 — Screening of the designed primers/probe of BEFV LFD-RPA assay.

(A) Agarose gel electrophoresis of RPA products generated using designed primers/probes. Lane M: molecular weight standard (DNA Marker 2000). Lane1 to 8 was designed primer and probe sets: F1-1/R1-1/1LF, F1-2/R1-2/1LF, F1-3/R1-3/1LF, F1-4/R1-4/1LF, 2F-1/2R-1/2LF, 2F-2/2R-2/2LF, 3F/3R/3LF and 4F/4R/4LF, respectively. Lane 9: positive control (supplied by Twist Amp nfo kit); Lane 10: negative control (DNase-free water); Specifically, Lane 7 was primers/probe set 3F/3R/3LF, and the expected size of the product was 235bp.

(B) Lateral-flow strip end-point analysis of RPA products generated by using designed primers/probe set 3F/3R/3LF. Lane 1 to 8: BEFV amplicons performed with RPA primer pair F1-1/R1-1/1LF, F1-2/R1-2/1LF, F1-3/R1-3/1LF, F1-4/R1-4/1LF, 2F-1/2R-1/2LF, 2F-2/2R-2/2LF, 3F/3R/3LF and 4F/4R/4LF, respectively. Lane 9: positive control (supplied by Twist Amp nfo kit); Lane 10: negative control (DNase-free water).