Table 1.

The sequences of primers and probes designed for screening in the study.

Primer/probe sets	Primers Name	Oligonucleotide sequences (5′-3′)	Product sizes (bp)
1	F1-1	5′-agagcttggtgtgaatacagaccttttgttgac-3′	251
	R1-1	5'-【Biotin】tcgaatttgatcaatttttgataatcctctatc-3′
	1LF	5'-【FAM】ttggaaacagcgccggcaggaggaagctc【dSpacer】ggaaataggctgaatgta【C3-spacer】-3′
2	F1-2	5′-attgaatggacataacatgtctgggaatcatg-3′	130
	R1-2	5'-【Biotin】caaatataatttagttggttccacgaagatcattc-3′
	1LF	5'-【FAM】ttggaaacagcgccggcaggaggaagctc【dSpacer】ggaaataggctgaatgta【C3-spacer】-3′
3	F1-3	5′-agagcttggtgtgaatacagaccttttgttgac-3′	169
	R1-3	5'-【Biotin】ttgataatcctctatcccctcatatagagatt-3′
	1LF	5'-【FAM】ttggaaacagcgccggcaggaggaagctc【dSpacer】ggaaataggctgaatgta【C3-spacer】-3′
4	F1-4	5′-agagcttggtgtgaatacagaccttttgttgac-3′	217
	R1-4	5'-【Biotin】gttctcttccacattatcatactccattacctcg-3′
	1LF	5'-【FAM】ttggaaacagcgccggcaggaggaagctc【dSpacer】ggaaataggctgaatgta【C3-spacer】-3′
5	2F-1	5′-cggaaataggctgaatgtaactctaaacggaatg-3′	178
	2R-1	5'-【Biotin】tttctcatcctcctcatacctaatcaagttctc-3′
	2LF	5'-【FAM】ctctatatgaggggatagaggattatcaaa【dSpacer】attgatcaaattcgagg【C3-spacer】-3′
6	2F-2	5′-cggaaataggctgaatgtaactctaaacggaatg-3′	189
	2R-2	5’-【Biotin】gtgcttgtaaaccaacctacaacagcagataaaac-3′
	2LF	5'-【FAM】ctctatatgaggggatagaggattatcaaa【dSpacer】attgatcaaattcgagg【C3-spacer】-3′
7	3F	5′- aggatggagaatggtggagtatagaaaacc -3′	235
	3R	5'-【Biotin】ctcgtgggagccaagtaagacatatctaatgt -3′
	3LF	5'-【FAM】aggatgcacacagatagaacagagtttgaa【dSpacer】aattagatattaaggc【C3-spacer】-3′
8	4F	5′-ctaaacggaatgatcttcgtggaaccaacta-3′	279
	4R	5'-【Biotin】cgtgcttgtaaaccaacctacaacagcagata-3′
	4LF	5'-【FAM】aatggagtatgataatgtggaagagaactt【dSpacer】attaggtatgaggagg【C3-spacer】-3′

Note: F: forward primer; R: reverse primer; LF: probe; FAM: Carboxyfluorescein; dSpacer: a tetrahydrofuran residue; C3-spacer: 3′-block.