Figure 1.

A representation of the PVX genome. (A) The five ORFs of the PVX genome are denoted by open boxes. The relative positions of the plus-strand probe (P1) and the minus-strand probe (P3) used for S₁ nuclease protection assays are indicated below and above the genome, respectively, with asterisks (∗) denoting the ³²P-labeled positions. (B) Within the 3′ NTR sequence, symbols are used to depict the hexanucleotide sequence (filled diamonds), the U-rich sequence (open circles), the first potential near-upstream element (NUE1; asterisk), and the second potential NUE (NUE2; plus sign). The relative positions of the primers P5 and P6 used for RNA sequencing and primer extension experiments are depicted below the 3′ NTR sequence.