Figure 5.

Effects of deletions extending from the 5′ region of the PVX 3′ NTR. (A) The sequence of the wild-type, pMON8453 3′ NTR is aligned with corresponding sequences from deletion mutants p71Δ10, p71Δ60 and Δint. The vertical arrow indicates the nucleotide mutated in pMON8453 to generate p71. Deleted sequences in p71Δ10, p71Δ60 and Δint are noted by lines. Different elements are illustrated above the wild-type sequence with symbols as described in the legend to Figure 1. (B) Protoplasts inoculated with replication-defective transcripts derived from p32 (p32), wild-type transcripts derived from pMON8453 (w.t.), and mutant transcripts (p71, p71Δ10, p71Δ60 and Δint) were analyzed at 40 hours post-inoculation by digestion with S₁ nuclease. The protected products were separated on denaturing 8% polyacrylamide gels; arrows indicate the direction of electrophoresis. The relative average values (±standard error) for minus-strand (−RNA) and plus-strand (+RNA) accumulation are noted at the outer edges of the autoradiograms.