Fig. 3.
shRNA-expressing plasmids remarkably inhibited TGEV RNA replication in different organs. (A) All the organs were sampled from the mini-pigs in group 5; (B) all the organs were sampled from the mini-pigs in group 2; M: 50 bp DNA ladder marker; A: mesenteric lymph node; B: lung; C: liver; D: jejunum; E: ileum; F: kidney; G: spleen. Aβ, Bβ, Cβ, Dβ, Eβ, Fβ, Gβ are products of β-actin gene expressed in these organs; AN, BN, CN, DN, EN, FN, GN are products of the N gene of TGEV in these organs. The TGEV N gene fragments, amplified successfully in all samples in group 2, were undetectable in group 5.