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Fluorescence quenching spectra of the P domain peptide at different concentrations of PB2 (a, 0 μM; b, 8.41 μM; c, 16.82 μM; d, 24.23 μM; e, 33.64 μM; f, 42.05 μM; g, 50.46 μM; h, 58.87 μM; i, 67.28 μM; j, 75.69 μM; k, 84.1 μM). The protein sample was excited at 280 nm using a slit-width of 2.5 nm. Emission scans were from 300 nm to 450 nm using a 5 nm slit-width. [P domain] = 8.41 μM, [PBS] = 10 mM, pH 7.4, 25 °C.
