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Stern-Volmer curves for the binding of PB2 (A: adding with high concentration 0–80 μM of PB2, B: adding with low concentration 0–35 μM of PB2) with the P domain, double-logarithm plot of the PB2 quenching effect on P domain fluorescence (C), and Van't Hoff plot for the interaction of P domain and B2 (D) at 298, 303, and 310 K. Conditions: [P domain] = 8.41 μM, [PBS] = 10 mM, pH 7.4, [PB2] = 8.41–84.1 μM.
