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(A) The ultraviolet–visible spectroscopy of P domain (8.41 μM) with PB2 at different concentrations (a, 0 μM; b, 8.41 μM; c, 16.82 μM; d, 24.23 μM; e, 33.64 μM; f, 42.05 μM; g, 50.46 μM; h, 58.87 μM)[PBS] = 10 mM, pH 7.4, 25 °C. (B) CD spectra of P domain with or without PB2. (C) Estimated secondary structural fractions of P domain with or without PB2 by K2D software. Conditions: [P domain] = 8.41 μM in buffer containing 10 mM PBS (pH 7.4), [PB2] = 0.1 mM, 25 °C.
