Fig. 2.

(A) Exploded view of five layers for constructing an integrated RT-PCR-ICS microdevice: from bottom to top, an ICS, a RTD wafer, a channel wafer, a monolithic PDMS membrane, and a glass manifold. (B) Schematics of an integrated RT-PCR-ICS microdevice: a pneumatic micropump (orange), a PCR chamber (green), a microfabricated RTD (yellow), a PDMS microvalve (violet), and an ICS (grey). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)