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Optimization of an ICS based colorimetric detection by tuning the length of the spacer between biotin and dUTP and the concentration ratio of dTTP and biotin–dUTP. The carbon number of the spacer was (A) 11, (B) 16, and (C) 18. The ratio of dTTP:biotin–dUTP was 1:0, 2:1, 4:1 and 8:1.
