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. 2019 Dec 12;140:103922. doi: 10.1016/j.micpath.2019.103922

Fig. 1.

Fig. 1

Identification of the isolated PEDV HM2017 strain (P8). (A) Cytopathic effects of the PEDV HM2017 strain on Vero cells at 36 h post-inoculation (hpi) (100× magnification). (a) Mock-inoculated Vero cells culture showing normal cells. (b) HM2017-inoculated Vero cells showing rounded and clustered cells. (B) Electron microscopy of the supernatants of the HM2017-inoculated Vero cells by using negative staining with phosphotungstic acid. Typical coronavirus-like particles were visible (Scale bar = 200 nm). (C) Detection of PEDV HM2017 strain in Vero cells by immunofluorescence (IF) staining using monoclonal antibody against spike protein of PEDV at 36 hpi (200× magnification). (a) IF staining of mock-inoculated Vero cells showing no IF-positive cells. (b) IF-stained cells were visible in HM2017-inoculated Vero cells.