Fig. 2.

Plaque purification and viral growth kinetic of PEDV HM2017 strain (P15). (A) Plaque purification of PEDV HM2017 strain. Monolayers of Vero cells were inoculated with PEDV HM2017 strain. After incubation for 2 h, the cells overlaid with 1.5% agarose. Plaques were stained with crystal violet at 48 hpi. (B) Viral growth kinetics in Vero cells. Vero cells were inoculated with PEDV HM2017 strain and PEDV CV777 strain at MOI = 0.01, respectively. Virus titers at different time points were determined and the 50% tissue culture infectious dose (TCID₅₀) was calculated. Asterisk (*) indicates a significant difference between PEDV HM2017 strain and PEDV CV777 strain (*, P < 0.05; **, P < 0.01).