Fig. 2.

Titers of antibody responses in immune sera of mice received either the tPA-D8L or wtD8L DNA vaccines. IgG antibody responses as measured by ELISA against either recombinant D8 antigen produced from 293T cells (A) or lysate of Vero cells infected with vaccinia virus WR strain (VACV) (B). Control groups of mice received either the empty DNA vector (Vector) or the vaccinia virus WR strain (WR). Each group included 5 mice and data shown are geometric means of the end titration titers for each group. (C) Titers of neutralizing antibodies for the same groups of mouse sera in an IMV-based, plaque reduction assay. Data shown are geometric means of neutralizing antibody titers which are the highest sera dilution that could inhibit 50% of virus infection in this assay.