Fig. 4.

Toxoid-specific IgG titer in second application or 365 days after first application. The hydrogel patch containing TT and DT (2 mg each/5 cm × 8 cm) was applied on the left brachial medial skin for 24 h. The second vaccination in 5 subjects was administered 140 days after first vaccination using the same procedures. Serum was collected on days 140 and 200 and anti-TT (A) or DT (B) IgG titer was determined by ELISA. Additionally, serum was collected from 6 subjects 365 days after the first vaccination and anti-TT (C) or DT (D) IgG titer was determined by ELISA. Open circle indicated each sample, and bar indicated geometric mean value.