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. 2018 Feb 27;38:7–12. doi: 10.1016/j.mcp.2018.02.004

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© 2018 Elsevier Ltd. All rights reserved.

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Fig. 4 — Intra-assay and inter-assay coefficients of variations (CV%) of the multiplex real-time PCR. Low (10⁴), medium (10⁶) and high concentration (10⁸) genome copies were conducted in the CPV-2 and its variants assay and the CPV-2a, 2b and 2c assay in triplicate. CVs of intra-assay and inter-assay were less than 2.5%.