Fig. 1.

Experimental infection of calves with ‘Hobi’-like pestivirus. Animals inoculated intranasally with strain Italy-1/10-1 were monitored for up to 28 days for the occurrence of fever (panel A) and variations in total WBC, lymphocyte and polymorphocyte counts (panel B). In addition, viremia and viral RNA shed in faeces and nasal secretions (panel C) and antibody responses (panel D) were determined. Temperatures are presented as median degrees Celsius (°C) and total WBC, lymphocyte, and polymorphocyte counts as median percentages of the cell counts determined at day 0. Viral RNA titres as determined by real-time RT-PCR are expressed as median copy numbers per μl of template. Antibody responses are presented as median virus neutralising (VN) titres and ELISA optical density (OD) values.