Fig. 5.
3Cpro induces PKR reduction at posttranslational levels. (A) PK-15 cells were transfected with increasing amounts of Flag-3C plasmids (0, 0.5, 1, 2 or 3 μg) for 36 h. The empty vector plasmids were used in the transfection process to ensure that the same amount of cells received the same amount of total plasmids. The expressions of PKR mRNA levels were detected by qPCR method. (B) PK-15 cells were transfected with vector plasmids or Flag-3C plasmids. The cells were collected at 0, 4, 8, 16 or 24 hpt and subjected to western blotting analysis. The PKR protein levels were then detected and compared. (C) PK-15 cells were treated with DMSO (solvent control) or CHX (100 μg/mL) for 0, 6, 12 or 18 h, the expression of PKR was detected by western blotting.