Table 2.
Genome organization and coding sequences annotation from the HAdV-B14 CHN strain.
| Region | Nucleotide locality | Gene product | Product length | TATA box | ATG site | Stop site | Poly (A) signal |
|---|---|---|---|---|---|---|---|
| ITR | 1–137 | ||||||
| E1A | 587–1166, 1251–1459 | 29.1 kDa | 262 | 494–501 | 587 | 1457 | 1518–1523 |
| E1A | 587–1073, 1251–1459 | 25.7 kDa | 231 | 494–501 | 587 | 1457 | 1518–1523 |
| E1A | 587–658, 1251–1355 | 6.5 kDa | 58 | 494–501 | 587 | 1353–1355 | 1518–1523 |
| E1B | 1629–2171 | 20 kDa | 180 | 1575–1581 | 1629 | 2169–2171 | 3471–3476 |
| E1B | 1934–3418 | 54.9 kDa | 494 | 1575–1581 | 1934 | 3416–3418 | 3471–3476 |
| IVa2 | 3985c–5031c | IVa2 protein | 348 | ND | 5607c | 3985c–3987c | 3963c–3968c |
| E2A | 21833c–23389c | DNA binding protein | 518 | ND | 23387c | 21833c | 21791c–21796c |
| E2B | 5088c–8660c, 13643c–13651c | DNA polymerase | 1122 | 13693c–13698c | 13649c | 5088c | ND |
| E2B | 8459–10420, 13643–13651 | pTP | 656 | 13693c–13698c | ND | ND | ND |
| IX | 3498–3917 | 14.2 kDa | 139 | 3416–3421 | 3501 | 3918 | 3942–3947 |
| E4 | 31801c–32052c | ORF6/7 | 141 | 34452c–34459c | 32940c–32942c | 31795c–31797c | 31778c–31783c |
| E4 | 32043c–32942c | ORF6; 34 kDa | 299 | 34452c–34459c | 32940c–32942c | 32043c–32045c | 31778c–31783c |
| E4 | 32845c–33213c | ORF4; 13 kDa | 122 | 34452c–34459c | 33211c–33213c | 32845c–32847c | 31778c–31783c |
| E4 | 33222c–33575c | ORF3; 13.5 kDa | 117 | 34452c–34459c | 33573c–33575c | 33222c–33224c | 31778c–31783c |
| E4 | 33572c–33961c | ORF2; 14.3 kDa | 129 | 34452c–34459c | 33959c–33961c | 33572c–33574c | 31778c–31783c |
| E4 | 34004c–34381c | ORF1; 14.2 kDa | 125 | 34452c–34459c | 34379c–34381c | 34004c–34006c | 31778c–31783c |
| L1 | 10668–11828 | 43.9 kDa | 386 | ND | 10668–10670 | 11826–11828 | 13624–13629 |
| L1 | 11854–13617 | pIIIa; 65.6 kDa | 587 | ND | 11854–11856 | 13615–13617 | 13624–13629 |
| L2 | 13699–15375 | Penton | 558 | ND | 13699–13701 | 15373–15375 | 17335–17340 |
| L2 | 15380–15958 | pVII | 192 | ND | 15380–15382 | 15956–15958 | 17335–17340 |
| L2 | 16001–17056 | pV | 351 | ND | 16001–16003 | 17054–17056 | 17335–17340 |
| L2 | 17085–17315 | pX | 76 | ND | 17085–17087 | 17313–17315 | 17335–17340 |
| L3 | 17396–18136 | pVI | 246 | ND | 17396–17398 | 18134–18136 | 21779–21784 |
| L3 | 18252–21089 | Hexon | 945 | ND | 18252–18254 | 21087–21089 | 21779–21784 |
| L3 | 21126–21755 | 23 kDa | 209 | ND | 21126–21128 | 21753–21755 | 21779–21784 |
| L4 | 23420–25858 | 100 kDa | 812 | ND | 23420–23422 | 25856–25858 | 27333–27338 |
| L4 | 25590–26165 | 22 kDa | 191 | ND | 25590–25592 | 26163–26165 | 27333–27338 |
| L4 | 26047–26439 | 33 kDa | 130 | ND | 25590–25592 | 26437–26439 | 27333–27338 |
| L4 | 26489–27172 | pVIII | 227 | ND | 26489–26491 | 27170–27172 | 27333–27338 |
| E3 | 27172–27489 | 11.7 kDa | 105 | 26854–26859 | 27172–27174 | 27487–27489 | 30596–30601 |
| E3 | 27443–27838 | 14.6 kDa | 131 | 26854–26859 | 27443–27445 | 27836–27838 | 30596–30601 |
| E3 | 27823–28323 | 18.4 kDa | 166 | 26854–26859 | 27823–27825 | 28321–28323 | 30596–30601 |
| E3 | 28343–28888 | 20.1 kDa | 181 | 26854–26859 | 28343–28345 | 28886–28888 | 30596–30601 |
| E3 | 28906–29457 | 20.8 kDa | 183 | 26854–26859 | 28906–28908 | 29455–29457 | 30596–30601 |
| E3 | 29501–29776 | 10.1 kDa | 91 | 26854–26859 | 29501–29503 | 29774–29776 | 30596–30601 |
| E3 | 29781–30185 | 14.9 kDa | 134 | 26854–26859 | 29781–29783 | 30183–30185 | 30596–30601 |
| E3 | 30178–30585 | 15 kDa | 135 | 26854–26859 | 30178–30180 | 30583–30585 | 30596–30601 |
| U | 30609c–30733c | U protein | 54 | ND | 30771c | 30609c | ND |
| ND | 102c–281c | Hypothetical protein | 141 | ND | 102c | 281c | ND |
| L5 | 30788–31759 | Fiber | 323 | ND | 30788–30790 | 31757–31759 | 31762–31767 |
| ITR | 34624c–34760c |
Coding sequences with spliced regions are indicated by double entries separated by “,” in the nucleotide locality column. Coding sequences transcribed from the complementary strand are designated by “c”. ND; not determined. p: protein.